NFKB p65 (phospho-Thr254) antibody

• Catalog Number: orb6507
• Category: Antibodies
• Description: NFKB p65 (phospho-Thr254) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human NFKBp65 around the phosphorylation site of Thr254 (FR(p-T)PP)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=2ug/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: phospho-NFKB p65 (Thr254)
• UniProt ID: Q04206
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: NF-kB p65 (phospho T254); p-NF-kB p65 (phospho T25
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of HL 60 cells using NFKB p65 (phospho-Thr254) antibody.

Western blot analysis of mouse lung tissue (Line 1) , mouse spleen cell lysate (Line 2) using NFKB p65 (phospho-Thr254) antibody.

Western blot analysis of extracts from spleen tissue(mo) using Phospho-NFKB p65(Thr254) antibody.