NFkB p65 antibody

• Catalog Number: orb344389
• Category: Antibodies
• Description: NFkB p65 antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 27F9.G4
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration: 0.98 mg/mL
• Dilution range: ELISA: 1:50,000-1:100,000, IHC: 1:200-1:600, IF: 1:5,000, WB: 1:1,000-1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-NF-kB p65 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography and showed a single band by IEP (immunoelectrophoresis) when tested with anti-mouse antibody. Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.
• Conjugation: Unconjugated
• UniProt ID: Q04206
• NCBI: 223468676
• Storage: Store NF-kB p65 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: mouse anti-NF-kB p65 Antibody, mouse anti-Rel A an
• Note: For research use only
• Application notes: Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 (Rel A) and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately. This product tested in WB, ICC, IHC, and IF.
• Expiration Date: 12 months from date of receipt.

References

Li, Ying et al. Pterostilbene pre-treatment reduces LPS-induced acute lung injury through activating NR4A1 Pharm Biol, 60, 394-403 (2022)

PubMed: 35271397

Applications

WB

Reactivity

Rat

Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020)

PubMed: 32050796

Applications

WB

Reactivity

Rat

Yang, Xifeng et al. Downregulation of miR‑181b inhibits human colon cancer cell proliferation by targeting CYLD and inhibiting the NF‑κB signaling pathway Int J Mol Med, 46, 1755-1764 (2020)

PubMed: 32901872

Applications

WB

Reactivity

Human

Western blot analysis of used to detect p65 using NFkB p65 antibody

Immunofluorescence analysis of Human Fibroblasts tissue using NFkB p65 antibody

Immunofluorescence analysis of Hela cells using NFkB p65 antibody

Immunofluorescence analysis of mouse endothelial cells using NFkB p65 antibody

Immunohistochemical staining of human tissues showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissu

Western blot analysis of HeLa whole cell lysate using NFkB p65 antibody