NFkB p105/p50/NFKB1 Antibody

• Catalog Number: orb215903
• Category: Antibodies
• Description: NFkB p105/p50/NFKB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human NFkB/NFKB1 p105/p50 recombinant protein (Position: M1-Q360). Human NFkB p105/P50 shares 93% amino acid (aa) sequence identity with mouse NFkB p105/P50.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 105356 MW
• UniProt ID: P19838
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Nuclear factor NF-kappa-B p105 subunit;DNA-binding
• Note: For research use only
• Application notes: WB: The detection limit for NFkB p105/P50 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HeLa cells using anti-NFkB p105/p50/NFKB1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of NFkB p105/p50/NFKB1 using anti-NFkB p105/p50/NFKB1 antibody.Lane 1:human Raji cell; 2:human SH-SY5Y cell; 3:human HeLa cell; 4:human Jurkat cell.

IF analysis of NFkB p105/p50/NFKB1 using anti-NFkB p105/p50/NFKB1 antibody. NFkB p105/p50/NFKB1 was detected in an immunocytochemical section of U2OS cells.