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Catalog Number | orb215904 |
---|---|
Category | Antibodies |
Description | NFkB p100/p52/NFKB2 MYD88 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Predicted Reactivity | Bovine |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human NFkB p100/p52 recombinant protein (Position: M1-R340). Human NFkB p100/p52 shares 96% amino acid (aa) sequence identity with mouse NFkB p100/p52. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 96749 MW |
UniProt ID | Q00653 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Myeloid differentiation primary response protein M Read more... |
Note | For research use only |
Application notes | WB: The detection limit for NFkB p100/p52 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
IHC analysis of NFkB p100 using anti-NFkB p100 antibody. NFkB p100 was detected in a paraffin-embedded section of human lung cancer tissue.
IHC analysis of NFkB p100 using anti-NFkB p100 antibody. NFkB p100 was detected in a paraffin-embedded section of human mammary cancer tissue.
IHC analysis of NFkB p100 using anti-NFkB p100 antibody. NFkB p100 was detected in a paraffin-embedded section of mouse intestine tissue.
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