NFKB inhibitor alpha Antibody

• Catalog Number: orb556701
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IKB alpha
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Human, Mouse, Porcine
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human IKB alpha. The exact sequence is proprietary.
• Concentration: 0.16 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 1XPBS, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 36
• Target: IKB alpha
• UniProt ID: P25963
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS (pH7), 1% rAlbumin, 20% Glycerol
• Alternative names: NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFK
• Note: For research use only
• Application notes: Positive control: BCL-1 , C2C12 , NIH-3T3 , HT-29 , 293T , A431 , HeLa , HepG2
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using IKB alpha antibody

Immunohistochemical staining of mouse kidney using IKB alpha antibody

Western blot analysis of Various whole cell using IKB alpha antibody

Western blot analysis of NIH-3T3 cells using IKB alpha antibody

Western blot analysis of BCL-1 whole cell lysate(Lane 1), C2C12 whole cell lysate(Lane 2) using IKB alpha antibody