NFIB Antibody

• Catalog Number: orb1524586
• Category: Antibodies
• Description: NFIB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 325 and 375
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NFIB
• UniProt ID: O00712
• NCBI: NP_005587.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: NFIB3; TGGCA-binding protein; Nuclear factor I/B;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NFIB by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-NFIB antibody orb1524586 used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human NFIB by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NFIB antibody orb1524586 used for IP at 6 µg/mg lysate.