NEUROD2 Antibody

SKU: orb1410084

Description

Mouse monoclonal antibody to NFIA

Research Area

Cell Biology, Epigenetics & Chromatin

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2c
• Clone No.: PCRP-NFIA-2C6
• Immunogen: Recombinant fragment of human NFIA protein corresponding to protein domain
• Target: NEUROD2
• Source/Expression System: Bioreactor
• Molecular Weight: 55kDa
• Purification: Protein A/G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Disclaimer: For research use only

Alternative Names

CCAAT-box-binding transcription factor; CTF; NF I/A; NF-I/A; NF1-A; NFI A; NFI L; NFIA; Nuclear factor 1 A type; Nuclear factor 1 A-type; TGGCA binding protein

Analysis of Protein Array containing more than 19000 full-length human proteins using NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow cytometric analysis of PFA-fixed HeLa cells. NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

Formalin-fixed, paraffin-embedded human lung stained with NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

SDS-PAGE Analysis Purified NFIA Mouse Monoclonal Antibody (PCRP-NFIA-2C6). Confirmation of Integrity and Purity of Antibody.

Quick Database Links

Gene Symbol

NEUROD2

UniProt

Q15784