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| Catalog Number | orb764248 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to NF kappa B-p65 (phospho-Thr254) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Thr254. AA range:221-270 |
| MW | 60kD |
| Tested applications | ELISA, IHC-P, IP, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-RELA antibody, anti-NFKB3 antibody, anti-Tran Read more... |
| Note | For research use only |
Western Blot analysis of various cells using Phospho-NFκB-p65 (T254) Polyclonal Antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr254) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with TNF-alpha, using NF-kappaB p65 (Phospho-Thr254) Antibody. The lane on the left is blocked with the phospho peptide.
