NFAT5 Antibody

• Catalog Number: orb1242766
• Category: Antibodies
• Description: NFAT5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse NFAT5.
• Concentration: batch dependent
• Dilution range: NFAT5 antibody can be used for detection of NFAT5 by ELISA at 1:62500. NFAT5 antibody can be used for detection of NFAT5 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 74 kDa
• Target: NFAT5
• UniProt ID: Q9JIH4
• NCBI: AAF79117
• Storage: For short periods of storage (days) store at 4°C. For longer periods of storage, store NFAT5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: NFAT5, CAG-8, CAG80, OREBP, nfatz, NFATL1, TonEBP,
• Note: For research use only
• Application notes: NFAT5 antibody can be used for detection of NFAT5 by ELISA at 1:62500. NFAT5 antibody can be used for detection of NFAT5 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Expiration Date: 12 months from date of receipt.

Antibody used in WB on HT-29 at 1:1000 (Lane 1: 20 ug HT 29 lysate blocked with 5% FBS Lane 2: 20 ug HT 29 lysate blocked with no FBS Lane 3: 20 ug HT-29 lyaste blocked with no PBS + 100 mM NaCl).

Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.