NF90/NF110 Antibody

• Catalog Number: orb1525426
• Category: Antibodies
• Description: NF90/NF110 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 50 and 100
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NF90/NF110
• UniProt ID: Q12906
• NCBI: NP_036350.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: nuclear factor associated with dsRNA; NF110b; NF90
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NF90 and NF110 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NF90/NF110 antibody orb1525426 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. NF90 and/or NF110 were also immunoprecipitated by rabbit anti-NF90/NF110 antibodies as well as anti-NF110 antibody, each of which recognizes a different epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).