NF90 Antibody

• Catalog Number: orb1524439
• Category: Antibodies
• Description: NF90 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 652 and 702
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NF90
• UniProt ID: Q12906
• NCBI: NP_004507.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: nuclear factor associated with dsRNA; NF110b; NF90
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NF90 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NF90 antibody orb1524439 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. NF90 was also immunoprecipitated by rabbit anti-NF90/NF110 antibodies, which recognize upstream epitopes. NF90 was not immunoprecipitated by rabbit anti-NF110 antibody. For blotting NF90 and NF110 (lower panel in B) , orb1525428 was used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Detection of human NF90 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NF90 (Cat. No. orb1524439) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB