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Catalog Number | orb1527729 |
---|---|
Category | Antibodies |
Description | NF-kappaB1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | between 918 and 968 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | NF-kappaB1 |
UniProt ID | P19838 |
NCBI | AAF35232.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | NFKB-p105; p50; p105; nuclear factor of kappa ligh Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human NF-kappaB1 by western blot. Samples: Whole cell lysate from HeLa (H; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NF-kappaB1 antibody orb1527729 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 1 minute.
Detection of human NF-kappaB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NF-kappaB1 antibody orb1527729 used for IP at 3 µg/mg lysate.
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