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Catalog Number | orb315636 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to NF-kappaB p100 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Porcine, Rat |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NF-kappaB p100. The exact sequence is proprietary. |
Dilution range | WB: 1-500:1000, IF/ICC: 1-100:500 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | NFKB2 |
Entrez | 4791, 18034 |
UniProt ID | Q00653, Q9WTK5 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti LYT10 antibody, anti Nuclear factor NF-kappa- Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of NF-kappaB p100 expression in Jurkat (A), MCF7 (B), HeLa (C) whole cell lysates. (Predicted band size: 96 kD; Observed band size: 100 kD)
Immunofluorescent analysis of NF-kappaB p100 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
IF, IH, IP, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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