NF kappa B-p100 antibody

• Catalog Number: orb765818
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NF kappa B-p100
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p100/p52. AA range:833-882
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-NFKB2 antibody, anti-LYT10 antibody, anti-Nuc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using NF-kappaB p100 Antibody. Primary Antibody was diluted at 1:1000 4°C over night, secondary antibody (Immunoway was diluted at 1:10000, 37°C 1 hour.

Immunofluorescence analysis of Hela cell. 1, NFκB-p100 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). ATG5 mouse Monoclonal Antibody (3C7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using NFκB-p100 Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of K562 cells using NFκB-p100 Polyclonal Antibody diluted at 1:1000.