Neutrophil Elastase/ELANE Antibody

• Catalog Number: orb381060
• Category: Antibodies
• Description: Neutrophil Elastase/ELANE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived mouse Elastase/ELANE/ELA2 recombinant protein (Position: S27-N265). Mouse Elastase/ELANE/ELA2 shares 92% amino acid (aa) sequence identity with rat Elastase/ELANE/ELA2.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 28648 MW
• UniProt ID: Q3UP87
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Neutrophil elastase;3.4.21.37;Elastase-2;Leukocyte
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Elastase/ELANE/ELA2 using anti-Elastase/ELANE/ELA2 antibody.Lane 1:mouse bone tissue; 2:mouse spleen tissue.

IHC analysis of Elastase/ELANE/ELA2 using anti-Elastase/ELANE/ELA2 antibody. Elastase/ELANE/ELA2 was detected in a paraffin-embedded section of rat spleen tissue.

IHC analysis of Elastase/ELANE/ELA2 using anti-Elastase/ELANE/ELA2 antibody. Elastase/ELANE/ELA2 was detected in a paraffin-embedded section of mouse spleen tissue.