Neutral Protease Dispase

SKU: orb420144

Description

Neutral Protease Dispase

Research Area

Epigenetics, Non-Animal

Images & Validation

• Application Notes:
  Neutral protease (Dispase

Key Properties

• Source: Bacillus polymyxa
• Biological Origin: Bacillus polymyxa
• Target: npr
• Purity: Neutral protease (Dispase) is purified and one unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using casein as the substrate. The enzyme is known to contain 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. Calcium has been detected in the purified protein and is believed to play a role in maintaining the structure and configuration, and preventing autolysis. Neutral protease is a non-specific metalloprotease. It cleaves fibronectin, collagen IV, and to a lesser extent collagen I, but it does not cleave collagen V or laminin. It hydrolyzes N-terminal peptide bonds of non-polar amino acid residues and may preferentially attack denatured and intercellular proteins with exposed hydrophobic amino acid residues. It is believed to bind one zinc ion and four calcium ions per subunit. Unlike other Bacillus species that produce neutral, alkaline, or a mixture of both proteases, Paenibacillus polymyxa is one of three species that produces only a neutral protease.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Stable at 2-8°C for 12 months. Aliquot and store at -20°C after reconstitution with water or commonly used balanced salt solutions or media. Stable over a wide pH range: 4.0-9.0, optimum pH 5.9-7.0. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: None. None
• Concentration: ≥ 4 units/mg
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Paenibacillus polymyxa, beta-amylase, alpha-amylase, trypsin, Bacillus subtilis, Bacillus thermoproteolyticus, Bacillus megaterium, Bacillus cereus, Streptomyces griseus, Aspergillus oryzae, Serratia, Bacillus polymyxa, Bacillolysin

Quick Database Links

Gene Symbol

npr

UniProt

P29148