Neuropilin 1 Antibody (monoclonal, 4G3F7)

• Catalog Number: orb1145780
• Category: Antibodies
• Description: Neuropilin 1 Antibody (monoclonal, 4G3F7)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4G3F7
• Tested applications: FC, ICC, IF, WB
• Reactivity: Human, Rat
• Isotype: Mouse IgG2b
• Immunogen: E.coli-derived human Neuropilin 1 recombinant protein (Position: K504-T827). Human Neuropilin 1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Neuropilin 1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5 μg/ml, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 120 kDa
• UniProt ID: O14786
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.

IF analysis of Neuropilin 1 using anti-Neuropilin 1 antibody. Neuropilin 1 was detected in an immunocytochemical section of A549 cells.

Western blot analysis of Neuropilin 1 using anti-Neuropilin 1 antibody.

Flow Cytometry analysis of U87 cells using anti-Neuropilin 1 antibody(Blue line).Isotype control antibody(Green line) was mouse IgG.Unlabelled sample(Red line) was also used as a control.