Neuron Specific Enolase Antibody [ENO2/1375]

• Catalog Number: orb1252204
• Category: Antibodies
• Description: Neuron Specific Enolase Antibody [ENO2/1375]
• Target: ENO2
• Clonality: Monoclonal
• Isotype: IgG2b
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.
• UniProt ID: P09104
• Tested applications: FC, IF, IHC, WB
• Application notes: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlIHC (FFPE): 0.1-0.2 ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: ENO2/1375
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neur
• Note: For research use only

IHC testing of FFPE human cerebellum with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE human pheochromocytoma with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

Western blot testing of human 1) Y79, 2) HeLa and 3) HepG2 cell lysate with NSE antibody (clone ENO2/1375). Predicted molecular weight ~47 kDa.

IHC testing of FFPE mouse pancreas with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE rat heart with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free NSE Antibody (clone ENO2/1375). Confirmation of Integrity and Purity of the Antibody.