ENO2 Antibody

SKU: orb1252151

Description

ENO2 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLImmunohistochemistry (FFPE): 0.1-0.2 µg/mL for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Neuron Specific Enolase antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: ENO2/1462
• Immunogen: Amino acids 416-433 of human NSE were used as the immunogen for this Neuron Specific Enolase antibody.
• Target: ENO2
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neurone-specific enolase, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE

Analysis of HuProt(TM) microarray containing more than 19000 full-length human proteins using Neuron Specific Enolase antibody (clone ENO2/1462). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human pheochromocytoma with NSE antibody (clone ENO2/1462). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE mouse pancreas with NSE antibody (clone ENO2/1462). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE rat heart with NSE antibody (clone ENO2/1462). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free Neuron Specific Enolase Antibody (clone ENO2/1462). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

ENO2

UniProt

P09104