Neurofilament heavy protein antibody

• Catalog Number: orb44466
• Category: Antibodies
• Description: Mouse Monoclonal to Neurofilament heavy protein.
• Clonality: Monoclonal
• Clone Number: NF-05
• Tested applications: ELISA, IHC-Fr, WB
• Reactivity: Human, Porcine, Rat
• Isotype: Mouse IgG1
• Immunogen: Pig brain neurofilament protein-enriched fraction after depolymerization of microtubules
• Concentration: 1 mg/ml
• Dilution range: ELISA: Capture antibody. Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: Neurofilament H
• Entrez: 4744
• UniProt ID: P12036
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Neurofilament heavy protein
• Note: For research use only
• Application notes: ELISA: Capture antibody. Western blotting: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of neurofilament heavy protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-05.

Western blotting analysis of human neurofilament H protein using mouse monoclonal antibody NF-05 on lysates of HEK-293 cell line, human brain lysate, and MCF-7 cell line (neurofilament non-expressing cell line; negative control) under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-neurofilament H monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Neurofilament H was detected around 180 kDa.