Neurofilament heavy protein antibody

• Catalog Number: orb44465
• Category: Antibodies
• Description: Mouse Monoclonal to Neurofilament heavy protein.
• Clonality: Monoclonal
• Clone Number: NF-01
• Tested applications: ICC, IHC-P, WB
• Reactivity: Mammal
• Isotype: Mouse IgG1
• Immunogen: Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Neurofilament H
• Entrez: 4744
• UniProt ID: P12036
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Neurofilament heavy protein
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-neurofilament heavy protein (NF-01).

Western blotting analysis of human neurofilament H protein using mouse monoclonal antibody NF-01 on lysates of HEK-293 cell line, human brain lysate, and MCF-7 cell line (neurofilament non-expressing cell line; negative control) under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-neurofilament H monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Neurofilament H was detected around 180 kDa.