Neurofilament Antibody (-Light) / NF-L / NEFL

• Catalog Number: orb385869
• Category: Antibodies
• Description: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. [RefSeq]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MSI27-1
• Tested applications: IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human neurofilament light chain protein was used as the immunogen for the Neurofilament antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Neurofilament antibody is available for research use only.
• UniProt ID: P07196
• Storage: Store the Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Neurofilament antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human cerebellum using Neurofilament (light chain) antibody