Neurofilament Antibody (-Heavy) / NF-H / NEFH

• Catalog Number: orb2642469
• Category: Antibodies
• Description: Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H).
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein A affinity
• Immunogen: Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant NF-H antibody.
• UniProt ID: P12036
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Application notes: The stated application concentrations are suggested starting points. Titration of the recombinant NF-H antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: NFHP-1R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant NF-H antibody is available for research use only.
• Note: For research use only

Western blot testing of human brain lysate with recombinant NF-H antibody (clone NFHP-1R). Expected molecular weight ~200 kDa.

IHC staining of FFPE human cerebellum with recombinant NF-H antibody (clone NFHP-1R). Required HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 10-20 min and allow to cool before testing.