NES / Nestin Antibody

• Catalog Number: orb1538817
• Category: Antibodies
• Description: NES / Nestin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.
• Concentration: 1 mg/ml
• Dilution range: ELISA (1:20000 - 1:80000), IHC (5 µg/ml), IHC-P (5 µg/ml), WB (1:500 - 1:3000)
• Conjugation: Unconjugated
• Target: NES / Nestin
• Storage: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
• Alternative names: NES, Nestin, Nbla00170
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B656 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and a panel of mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B656 was determined to be 5 ug/ml.
• Expiration Date: 12 months from date of receipt.