Nephrin/NPHS1 Antibody

• Catalog Number: orb389503
• Category: Antibodies
• Description: Nephrin/NPHS1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Nephrin recombinant protein (Position: V1084-V1241). Human Nephrin shares 79.9% and 75.3% amino acid (aa) sequence identity with mouse and rat Nephrin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, RatImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 134742 MW
• UniProt ID: O60500
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Nephrin;Renal glomerulus-specific cell adhesion re
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Nephrin using anti-Nephrin antibody.Lane 1:rat kidney tissue; 2:mouse kidney tissue.

IHC analysis of Nephrin using anti-Nephrin antibody. Nephrin was detected in a paraffin-embedded section of mouse kidney tissue.

IHC analysis of Nephrin using anti-Nephrin antibody. Nephrin was detected in a paraffin-embedded section of rat kidney tissue.