NEK9 (Phospho-T210) antibody

• Catalog Number: orb315630
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NEK9 (Phospho-T210)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T210 of human NEK9 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500:1000, IHC-P: 1-100:200, IF/ICC: 1-100:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NEK9
• Entrez: 217718, 91754
• UniProt ID: Q8TD19, Q8K1R7
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti KIAA1995 antibody, anti NEK8 antibody, anti N
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NEK9 (Phospho-T210) expression in THP1 (A), HepG2 (B), Hela (C) whole cell lysates. (Predicted band size: 107 kD; Observed band size: 120 kD)

Immunohistochemical analysis of NEK9 (Phospho-T210) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of NEK9 (Phospho-T210) staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.