NEIL1 antibody

• Catalog Number: orb18728
• Category: Antibodies
• Description: Goat polyclonal antibody to NEIL1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Bovine, Human, Porcine
• Dilution range: ELISA: 1:16000, IHC-P: 5-10µg/ml
• Conjugation: Unconjugated
• MW: 43.7
• Target: NEIL1 / NEH1
• Entrez: 79661
• Protein Sequence: DIPSLEPEGTSAS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NEIL1 antibody, anti NEH1 antibody, anti nei
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave an approx 60kDa band in Human Liver and Brain lysates at 1 μg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.7kDda according to NP_078884.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb18728 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.