NEFH Antibody (Monoclonal, N52)

• Catalog Number: orb18247
• Category: Antibodies
• Description: NEFH Antibody (Monoclonal, N52)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N52
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
• Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, -Western blot, 0.5ml, Human, mouse, rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 115378 MW
• UniProt ID: P16884
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Neurofilament heavy polypeptide;NF-H;200 kDa neuro
• Note: For research use only
• Application notes: Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
• Expiration Date: 12 months from date of receipt.

References

Zhao, Quanlai et al. Cytoprotective effects of myristicin against hypoxia‑induced apoptosis and endoplasmic reticulum stress in rat dorsal root ganglion neurons Mol Med Rep, 15, 2280-2288 (2017)

PubMed: 28260107

Immunohistochemical analysis of paraffin-embedded rat brain tissue using NEFH antibody

Western blot analysis of rat brain tissue lysates (Lane1), mouse brain tissue lysates (Lane2) using NEFH antibody