NEDD1 antibody

• Catalog Number: orb19382
• Category: Antibodies
• Description: Goat polyclonal antibody to NEDD1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 72
• Target: NEDD1
• Entrez: 121441
• Protein Sequence: NQTRNSEKFEKPEN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NEDD1 antibody, anti neural precursor cell ex
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75kDa band observed in lysates of cell line HeLa (calculated MW of 72.0kDa according to NP_690869.1). Recommended concentration: 1-3 μg/ml. An additional band was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide and likely represents the mature form. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19382 (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.