NEDD-9/CASL/HEF1 Antibody

• Catalog Number: orb1733239
• Category: Antibodies
• Description: NEDD-9/CASL/HEF1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 150 and 200
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1000, IP: 6 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NEDD-9/CASL/HEF1
• UniProt ID: Q14511
• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: CRK-associated substrate-related protein; Cas scaf
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 µg) from OVCAR-8, U-251MG, A-549, and UACC-257 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (orb1733239) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and C2C12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (orb1733239) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human NEDD-9/CASL/HEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (orb1733239) used for IP at 6 µl/mg lysate.