Nebulette antibody

• Catalog Number: orb1324
• Category: Tools
• Description: Nebulette antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Human, Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Nebulette (321-420/1014 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 116 kDa
• Target: Nebulette
• UniProt ID: O76041
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Actin binding Z disk protein; bA56H7.1; LIM nebule
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraffin-embedded mouse heart tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). Nebulette antibody at 1:200 dilution with overnight incubation at 4 degrees