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    NEAS/SPTAN1 Antibody

    Catalog Number: orb865468

    DispatchUsually dispatched within 5-10 working days
    $ 520.00
    Catalog Numberorb865468
    CategoryAntibodies
    DescriptionNEAS/SPTAN1 Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, FC, ICC, IF, IHC, WB
    ReactivityHuman, Mouse, Rat
    IsotypeRabbit IgG
    ImmunogenE.coli-derived human NEAS/SPTAN1 recombinant protein (Position: E1916-S2472).
    ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Dilution rangeWestern blot, 0.25-0.5 μg/ml, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human
    Form/AppearanceLyophilized
    ConjugationUnconjugated
    MW285 kDa
    UniProt IDQ13813
    StorageAt -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
    NoteFor research use only
    Application notesTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Expiration Date12 months from date of receipt.
    NEAS/SPTAN1 Antibody

    Western blot analysis of NEAS/SPTAN1 using anti-NEAS/SPTAN1 antibody (orb865468). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEAS/SPTAN1 antigen affinity purified polyclonal antibody (Catalog # orb865468) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for NEAS/SPTAN1 at approximately 285 kDa. The expected band size for NEAS/SPTAN1 is at 285 kDa.

    NEAS/SPTAN1 Antibody

    IHC analysis of NEAS/SPTAN1 using anti-NEAS/SPTAN1 antibody (orb865468). NEAS/SPTAN1 was detected in a paraffin-embedded section of human hashimoto thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEAS/SPTAN1 Antibody (orb865468) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

    NEAS/SPTAN1 Antibody

    IHC analysis of NEAS/SPTAN1 using anti-NEAS/SPTAN1 antibody (orb865468). NEAS/SPTAN1 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEAS/SPTAN1 Antibody (orb865468) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

    NEAS/SPTAN1 Antibody

    IHC analysis of NEAS/SPTAN1 using anti-NEAS/SPTAN1 antibody (orb865468). NEAS/SPTAN1 was detected in a paraffin-embedded section of human large B cell tumor of testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEAS/SPTAN1 Antibody (orb865468) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

    NEAS/SPTAN1 Antibody

    IHC analysis of NEAS/SPTAN1 using anti-NEAS/SPTAN1 antibody (orb865468). NEAS/SPTAN1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEAS/SPTAN1 Antibody (orb865468) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

    NEAS/SPTAN1 Antibody

    IHC analysis of NEAS/SPTAN1 using anti-NEAS/SPTAN1 antibody (orb865468). NEAS/SPTAN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEAS/SPTAN1 Antibody (orb865468) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

    NEAS/SPTAN1 Antibody

    IF analysis of NEAS/SPTAN1 using anti-NEAS/SPTAN1 antibody (orb865468). NEAS/SPTAN1 was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NEAS/SPTAN1 Antibody (orb865468) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    NEAS/SPTAN1 Antibody

    Flow Cytometry analysis of CACO-2 cells using anti-NEAS/SPTAN1 antibody (orb865468). Overlay histogram showing CACO-2 cells stained with orb865468 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEAS/SPTAN1 Antibody (orb865468, 1 μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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