NDUFC2 Antibody

• Catalog Number: orb1265010
• Category: Antibodies
• Description: NDUFC2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit Ig
• Immunogen: This NDUFC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-39 amino acids from the N-terminal region of human NDUFC2.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 14 kDa
• Target: NDUFC2
• UniProt ID: O95298
• NCBI: O95298
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: NADH dehydrogenase [ubiquinone] 1 subunit C2, Comp
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. kidney section using NDUFC2 Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of HepG2 cells stained with NDUFC2 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of HepG2 cells using NDUFC2 Antibody (N-term) (green) compared to an isotype control of rabbit IgG (blue). AP20601a was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded R. kidney section using NDUFC2 Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from Hela, HepG2, U-87 MG cell line, human kidney, mouse kidney and rat kidney tissue lysate (from left to right), using NDUFC2 Antibody at 1:1000 at each lane.