NDUFA5 Antibody

• Catalog Number: orb1274502
• Category: Antibodies
• Description: NDUFA5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 13kDa
• Target: NDUFA5
• UniProt ID: Q16718
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: B13, CI-13KD-B, CI-13kB, NUFM, UQOR13, NADH dehydr
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts from normal (control) and NDUFA5 knockout (KO) HeLa cells, using NDUFA5 antibody (orb1274502) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human tonsil using NDUFA5 antibody (orb1274502) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using NDUFA5 antibody (orb1274502) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using NDUFA5 antibody (orb1274502) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of NIH-3T3 cells using NDUFA5 Polyclonal Antibody (orb1274502) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.