NDE1 Antibody

• Catalog Number: orb1527457
• Category: Antibodies
• Description: NDE1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Immunogen: between 50 and 100
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-NDE1 antibody A301-974A.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NDE1
• UniProt ID: Q9NXR1
• NCBI: NP_060138.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: nuclear distribution protein nudE homolog 1; nudE
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NDE1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NDE1 antibody orb1527457 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NDE1 was also immunoprecipitated by rabbit anti-NDE1 antibodies, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).