NCR3LG1 Antibody

• Catalog Number: orb1671607
• Category: Antibodies
• Description: NCR3LG1 Antibody
• Clonality: Recombinant
• Clone Number: 17B1.3
• Tested applications: Crystallography, FC, IF
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Immunised mice with extracellular domain of human B7-H6 and selected on the basis of specific reactivity by P815.B7-H6, but not with P815.B7-H1.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: NCR3LG1
• UniProt ID: Q68D85
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: B7H6, B7 homolog 6, Natural cytotoxicity triggerin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-B7-H6 antibody 17B1.3. Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of 17B1.3 (orb1671607 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed K562 cells with anti-B7-H6 antibody 17B1.3. Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric r version of 17B1.3 (orb1671607) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671607- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.