NCL Antibody

• Catalog Number: orb1251982
• Category: Antibodies
• Description: NCL Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NPC23-2
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Recombinant human protein was used as the immunogen for the Nucleolin antibody.
• Concentration: 0.2 mg/mL
• Dilution range: IF: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlWestern blot: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the Nucleolin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NCL
• UniProt ID: P19338
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Nucleolin, Protein C23, NCL
• Note: For research use only
• Application notes: IF: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlWestern blot: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the Nucleolin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil with Nucleolin antibody (clone NPC23-2).

IHC testing of FFPE human ovarian carcinoma with Nucleolin antibody (clone NPC23-2).

Flow cytometry testing of human 293 cells and Alexa Fluor 488-labeled Nucleolin antibody (green).

Western blot testing of Nucleolin antibody and A431 lysate. Predicted molecular weight ~77 kDa.