NCKAP5L Antibody

• Catalog Number: orb1520243
• Category: Antibodies
• Description: NCKAP5L Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1025 and 1075
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 50-100 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NCKAP5L
• UniProt ID: Q9HCH0
• NCBI: Q9HCH0.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: centrosomal protein of 169 kDa; nck-associated pro
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse NCKAP5L by western blot. Samples: Whole cell lysate (10 µg) from K-562, Hep-G2, LNCaP, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCKAP5L antibody (orb1520243) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human NCKAP5L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCKAP5L antibody (orb1520243) used for IP at 1 µg per reaction.