NCK2 antibody

• Catalog Number: orb378194
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NCK2.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human NCK2
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NCK2
• Entrez: 8440, 17974
• UniProt ID: O55033, O43639
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: GRB4; Cytoplasmic protein NCK2; Growth factor rece
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NCK2 expression in HEK293T (A), mouse lung (B), rat brain (C) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 43 kD)

Immunohistochemical analysis of NCK2 staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.