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NBR1 monoclonal antibody (M05), clone 5C3

Catalog Number: orb2292629

DispatchUsually dispatched within 5-10 working days
$ 700.00
Catalog Numberorb2292629
CategoryAntibodies
DescriptionMouse monoclonal antibody raised against a partial recombinant NBR1.
ClonalityMonoclonal
Species/HostMouse
IsotypeIgG2a Kappa
ConjugationUnconjugated
ReactivityHuman
Buffer/PreservativesIn 1x PBS, pH 7.4
ImmunogenNBR1 (NP_005890, 2 a.a. ~ 96 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Protein SequenceEPQVTLNVTFKNEIQSFLVSDPENTTWADIEAMVKVSFDLNTIQIKYLDEENEEVSINSQGEYEEALKMAVKQGNQLQMQVHEGHHVVDEAPPPV
Tested applicationsELISA, WB
Clone Number5C3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NoteFor research use only
NCBINP_005890
NBR1 monoclonal antibody (M05), clone 5C3

Detection limit for recombinant GST tagged NBR1 is approximately 0.03 ng/ml as a capture antibody.

NBR1 monoclonal antibody (M05), clone 5C3

Western Blot analysis of NBR1 expression in transfected 293T cell line by NBR1 monoclonal antibody (M05), clone 5C3. Lane 1: NBR1 transfected lysate (107.4 KDa). Lane 2: Non-transfected lysate.

NBR1 monoclonal antibody (M05), clone 5C3

Western blot analysis of NBR1 over-expressed 293 cell line, cotransfected with NBR1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NBR1 monoclonal antibody (M05), clone 5C3 (Cat # orb2292629). GAPDH (36.1 kDa) used as specificity and loading control.

NBR1 monoclonal antibody (M05), clone 5C3

Western Blot detection against Immunogen (36.19 KDa).