NBEA Antibody

• Catalog Number: orb1247215
• Category: Antibodies
• Description: NBEA Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DFNRWHYEHQNRY
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 300kDa band observed in lysates of transfected HEK293 and endogenously in lysates of insulinoma cell line B-TC3 (calculated MW of 327kDa according to NP_056493.3). Data from a previous goat. Data kindly provided by Professor John Creemers, K.U. Leuven, Belgium. Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows textured cytoplasm staining in the neuronal cell bodies. Recommended concentration: 5-10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NBEA
• UniProt ID: Q8NFP9
• NCBI: NP_001191126.1, NP_056493.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NBEA, neurobeachin, RP11-270C18.1, BCL8B, FLJ10197
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 300kDa band observed in lysates of transfected HEK293 and endogenously in lysates of insulinoma cell line B-TC3 (calculated MW of 327kDa according to NP_056493.3). Data from a previous goat. Data kindly provided by Professor John Creemers, K.U. Leuven, Belgium. Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows textured cytoplasm staining in the neuronal cell bodies. Recommended concentration: 5-10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247215 (1 ug/ml) staining of 1) untransfected HEK293T cells 2) HEK293T cells transfected with mouse NBEA. Detected by chemiluminescence.

orb1247215 (5 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.