NAPSA Antibody

SKU: orb1252434

Description

NAPSA Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLWestern blot: 1-2 µg/mLImmunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Napsin A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: NAPSA/1239
• Immunogen: A recombinant fragment from the human protein was used as the immunogen for the Napsin A antibody.
• Target: NAPSA
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Napsin-A, 3423-, Aspartyl protease 4, ASP4, Asp 4, Napsin-1, TA01/TA02, NAPSA, NAP1, NAPA

Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with Napsin-A antibody (NAPSA/1239).

Western blot of 1) K562 and 2) HEK293 cell lysates using Napsin-A antibody (NAPSA/1239)

SDS-PAGE Analysis of Purified, BSA-Free Napsin A Antibody (clone NAPSA/1239). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

NAPSA

UniProt

O96009