NAPSA Antibody

• Catalog Number: orb1252434
• Category: Antibodies
• Description: NAPSA Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NAPSA/1239
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: A recombinant fragment from the human protein was used as the immunogen for the Napsin A antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Napsin A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NAPSA
• UniProt ID: O96009
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Napsin-A, 3423-, Aspartyl protease 4, ASP4, Asp 4,
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Napsin A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with Napsin-A antibody (NAPSA/1239).

Western blot of 1) K562 and 2) HEK293 cell lysates using Napsin-A antibody (NAPSA/1239)

SDS-PAGE Analysis of Purified, BSA-Free Napsin A Antibody (clone NAPSA/1239). Confirmation of Integrity and Purity of the Antibody.