NAMPT Antibody

• Catalog Number: orb1272636
• Category: Antibodies
• Description: NAMPT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human Visfatin. Human Visfatin specific antibody was purified by affinity chromatography employing an immobilized Human Visfatin matrix.
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: NAMPT
• UniProt ID: P43490
• NCBI: P43490
• Storage: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: VF, PBEF, PBEF1, VISFATIN, 1110035O14Rik, Nicotina
• Note: For research use only
• Application notes: ELISA:Sandwich:To detect Human Visfatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with BIorbyt’s Biotinylated Anti-Human Visfatin (38-282) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Visfatin.Western Blot:To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Human Visfatin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Visfatin (orb1272625) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Visfatin.

To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human ovary malignant adeoncarcinoma. The recommended concentration is 7.5ng/mL -15ng/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

This antibody stained formalin-fixed, paraffin-embedded sections of human ovary malignant adeoncarcinoma. The recommended concentration is 7.5ng/mL -15ng/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.