Nalp12/Nlrp12 Antibody

• Catalog Number: orb1248314
• Category: Antibodies
• Description: Nalp12/Nlrp12 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Rat
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: C-KPGTPTFKVPANQR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Mouse Liver and Spleen lysates (calculated MW of 119kDa according to NP_001028603.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Jéru et al, Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1614-9.PMID: 18230725). Recommended concentration: 1-3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 100kDa
• Target: Nalp12 / Nlrp12
• UniProt ID: E9Q5R7
• NCBI: NP_001028603.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Nlrp12, NLR family, pyrin domain containing 12, Na
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Mouse Liver and Spleen lysates (calculated MW of 119kDa according to NP_001028603.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Jéru et al, Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1614-9.PMID: 18230725). Recommended concentration: 1-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248314 (1 ug/ml) staining of Mouse Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.