NADPH oxidase 4/NOX4 Antibody

• Catalog Number: orb97055
• Category: Antibodies
• Description: NADPH oxidase 4/NOX4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, IHC-Fr, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse NADPH oxidase 4 (561-578aa NRNNSYGTKFEYNKES), identical to the related rat sequence and different from the related human sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 66519 MW
• UniProt ID: Q9JHI8
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: NADPH oxidase 4;1.6.3.-;Kidney oxidase-1;KOX-1;Kid
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Rat Kidney Tissue Lysate, Lane 2: Rat Cardiac Muscle Tissue Lysate, Lane 3: Rat Spleen Tissue Lysate, Lane 4: HELA Cell Lysate, Lane 5: 293T Cell Lysate, Lane 6: MCF-7 Cell Lysate, Lane 7: SMMC Cell Lysate using NOX4 antib

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Kidney Tissue using NOX4 antibody