N6022

SKU: orb1300362

Description

N6022 is a potent, selective, and reversible inhibitor of GSNOR with an IC50 of 8 nM. It is a valuable research tool for studying nitric oxide signaling in models of asthma, pulmonary hypertension, and other inflammatory conditions in both cellular and animal studies.

Research Area

Metabolism Research

Key Properties

• CAS Number: 1208315-24-5
• MW: 414.46
• Purity: 98.32% (May vary between batches)
• Formula: C₂₄H₂₂N₄O₃
• SMILES: Cc1cc(ccc1-n1c(CCC(O)=O)ccc1-c1ccc(cc1)-n1ccnc1)C(N)=O
• Target: GSNOR
• Solubility: DMSO:122.5 mg/mL (295.57 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.83 mM)

Bioactivity

• Target IC50:
  GSNOR:8 nM
• In Vivo:
  N6022 treats rats in 50 mg/kg leading to a slight increase in the incidence of granulomas. In serum, N6022 remained in solution is up to 5 mg/mL.
• In Vitro:
  N6022 binds to rat plasma proteins in a concentration-dependent manner. At lower drug concentrations (20 μM), N6022 show more effect on ATP than on GSH. N6022 (IC50 of 8 nM and a Ki of 2.5 nM) binds in the GSNO substrate binding pocket like a competitive inhibitor. N6022 is uncompetitive with cofactors NAD+ and NADH.
• Cell Research:
  N6022 is dissolved in 5% DMSO. N6022 is tested using a rat hepatoma (H4IIE) cell line whereby cells are seeded into 96-well plates and cultured in medium containing 20% bovine serum. Following an equilibration period of 48 h, the cells are treated with N6022 (5% DMSO vehicle) at concentrations of 0, 1, 5, 10, 20, 50, 100, and 300 μM for 24 h at 37°C in 5% CO2. Camptothecin and rotenone are included as positive controls. The cell supernatant or the cells themselves are harvested for biochemical analysis.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GSNOR, N6022, N-6022, inhibit, Inhibitor, N 6022, S-nitrosoglutathione reductase, S-nitrosoglutathione reductase (GSNOR)

Compound Identifiers

PubChem CID

44623946