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Catalog Number | orb1527335 |
---|---|
Category | Antibodies |
Description | Myosin-10 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse |
Immunogen | between 1955 and 1976 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-Myosin-10 antibody A302-042A. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Myosin-10 |
UniProt ID | P35580 |
NCBI | NP_005955.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | nonmuscle myosin II heavy chain-B; Non-muscle myos Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse Myosin-10 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50µg) cells. Antibodies: Affinity purified rabbit anti-Myosin-10 antibody orb1527335 used for WB at 1 µg/ml. Detection: Chemiluminescence with exposure time of 10 seconds.
Detection of human Myosin-10 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Myosin-10 antibody orb1527335 used for IP at 3 µg/mg lysate.
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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