MYOD1 (pS200) antibody

• Catalog Number: orb665479
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MYOD1 (Phospho-S200)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat, Sheep, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S200 of human MYOD1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MYOD1
• Entrez: 17927, 4654, 337868
• UniProt ID: P15172, Q02346, P10085
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-BHLHC1 antibody, anti-MYF3 antibody, anti-MYO
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MYOD1 (Phospho-S200) expression in EC9706 (A), mouse muscle (B) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 45 kD)

Immunohistochemical analysis of MYOD1 (Phospho-S200) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.