MyoD1 antibody

• Catalog Number: orb108820
• Category: Antibodies
• Description: Purified monoclonal antibody 5.2F in phosphate buffered saline with 0.08% sodium azide
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5.2F
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2a
• Immunogen: Recombinant mouse MyoD1 protein.
• Concentration: See vial for concentration
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• UniProt ID: P15172, P10085, P16075, Q02346
• Source: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.
• Storage: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
• Alternative names: Myoblast determination protein 1, Myogenic factor
• Note: For research use only
• Application notes: This antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 µg/mg of protein lysate), Western blotting (1 µg/ml) and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 µg/ml). These working concentrations are merely an indication. Optimal working concentrations should, however, be evaluated by serial dilutions by the customer.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MyoD1 protein using MyoD1 antibody

Immunohistochemical staining of human rhabdomyosarcoma using MyoD1 antibody